Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in the Registration Statement of Enzo Biochem, Inc. on Form S-8 (No. 333-197028 and 333-236958) of our report dated June 29, 2023 on our audits of the financial statements of the Enzo Biochem, Inc. Salary Reduction Profit Sharing Plan as of December 31, 2022 and 2021 and for the year ended December 31, 2022 and supplemental schedule as of December 31, 2022, which report is included in this Annual Report on Form 11-K to be filed on or about June 29, 2023.

/s/ EisnerAmper LLP

EISNERAMPER LLP

New York, New York

June 29, 2023